center for systems

& synthetic biology

an NIGMS national systems biology center


Structure to systems analysis of RNA polymerase II

August 15, 2013

Center investigator Nevan Krogan and colleagues have taken E-MAP technology to the next level by examining the genetic interaction space of point mutant alleles of multifunctional genes.

In new work published in Cell, Braberg et al. generated a point mutant E-MAP of RNA polymerase II (RNAPII) in S. cerevisiae, which allowed the assignment of specific genetic relationships and functions down to individual residues and domains of RNAPII. Based on the data, the authors could propose a model in which transcription start and splicing are intimately coupled with RNAPII elongation. Because many genes, especially essential ones, are multifunctional, the point mutant E-MAP technique could be a powerful new approach for high-resolution interrogation of other multifunctional machines. Read press highlights at UCSF news and Bionews Texas.