center for systems

& synthetic biology

an NIGMS national systems biology center


Mapping genetic interactions in mammalian cells

February 13, 2013

New work from Center investigator Nevan Krogan and colleagues published in Nature Methods takes advantage of recent developments in RNAi technology to develop a new platform for mapping genetic interactions in mammalian cells.

Roguev et al. used pairwise gene knockdown to create genetic interaction maps for 130 mammalian genes involved in chromatin regulation. Ultimately, this technology could be used to genetically interrogate functionally related sets of genes in a a variety of mammalian cell types.